Figure 3.

Male Mating Potency and Efficiency Rescue in PEPCK-Deficient Mutants

(A) Mating potency of 24-h pck-1(0); pck-2(0) adult males expressing transgenic tissue-specific-expressed pck-1::YFP or pck-2::YFP. Numbers of males assayed are listed at the bottom, and % potency values are listed at the top of the bars. p Values were determined using Fisher's exact test. Error bars represent the 95% confidence intervals.

(B–E) Copulation endurance assay. x axis depicts genotype and growth condition. y axis depicts the number of females that were impregnated. The data for each condition constitutes 10 trials with one male per -trial (see also Figure S6). Each symbol represents a single male. The gray window denotes matings wherein animals were incubated with 1% glucose. Bars and whiskers represent mean and standard deviation. p values were determined using the Mann-Whitney test. (B) Number of females impregnated in the first 12 h after adult molt. (C) Number of females impregnated between 12 and 24 h. (D) Number of females impregnated between 24 and 36 h. (E) Number of females impregnated between 36 and 48 h.

This figure is related to Figure S6.