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. 2020 Mar 19;23(4):100990. doi: 10.1016/j.isci.2020.100990

Figure 6.

Figure 6

SDHA-1 Inhibition on PCK-2 Expression in Hermaphrodites and Males

(A) Fluorescence micrographs showing a PCK-2::YFP male expressing sdha-1 and SL2 trans-spliced CFP from the sdha-1 promoter.

(B) Yellow emission fluorescence intensity of whole-animal PCK-2::YFP during different adult ages. Fluorescence values are in arbitrary units (A.U). Symbols represent individual wild-type, sdha-1(rg550), and transgenic sdha-1(rg550) transgenic males that express sdha-1(+) from its native promotor (sdha-1 promoter) or a muscle promoter (unc-103A promoter). For each stage, the number of animals quantified is 10 per strain. Each symbol represents a single male of the specified age. Bars and whiskers represent mean and standard deviation. p values were determined using the Mann-Whitney test.

(C) Fluorescence micrograph showing epidermal CFP-expressing mitochondria from a heat-shocked hermaphrodite that expresses CFP fused to SDHA-1.

(D) Yellow emission fluorescence intensity of whole animal PCK-2::YFP in 18- to 24-h adult males. Males were heat shocked at L4 stage to either induce transgenic sdha-1:CFP expression or test if heat shock artificially changed PCK-2::YFP levels. Fluorescence values are in arbitrary units (A.U). Symbols represent individual wild-type sdha-1(rg550) and transgenic wild-type and sdha-1(rg550) males that express sdha-1::CFP from a heat shock promoter (hsp-16 promoter). Numbers of animals quantified are listed at the bottom of the graph. Bars and whiskers represent mean and standard deviation. p values were determined using the Mann-Whitney test.