Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Mar 3;21(5):2171–2181. doi: 10.3892/mmr.2020.11010

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Lin et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Figure 3. — Nec-1 attenuates the ROS response to LPS-induced ALI in vivo. (A) Western blotting of lysates in the indicated mouse lung tissues was used to estimate the expression levels of ROS-associated proteins (NRF2 and HO-1). (B) Semi-quantification of the protein expression levels of NRF2 and HO-1. Representative images of at least three independent experiments are shown. (C) mRNA expression levels of NRF2 and HO-1 in lung tissues from sham-operated, LPS-ALI and Nec-1 + LPS-ALI mice were detected by reverse transcription-quantitative PCR and were normalized to GAPDH. (D) Levels of SOD2 and MDA in the lung tissue homogenates were detected using commercial kits. (E) Intracellular ROS production of the indicated lung cells was analyzed by DCFH-DA staining and flow cytometry. Data are presented as the mean ± SEM from at least three independent experiments (n=5 mice/group). *P<0.05, as indicated. ALI, acute lung injury; HO-1, heme oxygenase-1; LPS, lipopolysaccharide; MDA, malondialdehyde; Nec-1, necrostatin-1; NRF2, nuclear factor erythroid 2-related factor 2; SOD2, superoxide dismutase.