Table 1.
Limits of detection (LoD95) for respiratory targets
| Viral organism | LoD95 (pfu/mL) |
|---|---|
| Adenovirus type 3 | 79 |
| Adenovirus type 4 | 71 |
| Human bocavirus | 86 (cop/μL) |
| Coxsackievirus A | 1 × 103 |
| Coxsackievirus B | 1 × 102 |
| Echovirus | 1 |
| Influenza A H1N1-09 | 7 |
| Coronavirus 229E | 6 |
| Coronavirus HKU1 | 87 (cop/μL) |
| Coronavirus NL63 | 1 |
| Coronavirus OC43 | 7 |
| Influenza A H3N2 | 1 |
| Influenza B | 1 |
| Parainfluenza virus type 1 | 10 |
| Parainfluenza virus type 2 | 6 |
| Parainfluenza virus type 3 | 100 |
| Parainfluenza virus type 4A | 88 |
| Rhinovirus | 1 |
| Respiratory syncytial virus A | 1 |
| Respiratory syncytial virus B | 1 |
| Human metapneumovirus A | 562 |
| Human metapneumovirus B |
316 |
| Bacterial organism |
LoD95 (cfu/mL) |
| Acinetobacter baumannii | 7.5 × 104 |
| Chlamydophila pneumoniae | 100 |
| Haemophilus influenzae (nonspecific: types A-F) | 1 × 103-1 × 104 |
| H influenzae type B | 1 × 103-1 × 104 |
| Klebsiella pneumoniae | 1 × 103 |
| Legionella pneumophila | 750 |
| Mycoplasma pneumoniae | 10 |
| Moraxella catarrhalis | 100 |
| Neisseria meningitidis | 72 |
| Pseudomonas aeruginosa | 1 × 103 |
| Bordetella pertussis | 6.8 × 104 |
| Staphylococcus aureus | 100 |
| Streptococcus pneumoniae | 464 |
| Str pyogenes | 1 × 104 |
| Panton-Valentine leukocidin (cytotoxin) | 3.20 × 104 |
| Methicillin resistance (antibiotic resistance) | 100 |
NOTE. Analytical sensitivities were determined following Clinical and Laboratory Standards Institute guidelines. At the concentrations listed, the corresponding genetic target is a true positive result 95% of the time. The Supplemental Data describes the analytical validation procedures, including the measurement of >95% test specificity.
cfu, colony forming units; cop, copies; pfu, plaque forming units; LoD95, 95% Limit of Detection.