Table 4.

Study GLP1536: efficacy of 0.5% sodium hypochlorite with color additive after 10 minutes ± 5 seconds contact time to human coronavirus strain 229E dried on inanimate surface

					Color additive: Lot HLT 3-16-HLTS1				Color additive: Lot HLT 3-16-HLTS1
					0.5% sodium hypochlorite: Lot E616050				0.5% sodium hypochlorite: Lot E616060
Dilution	Dried Virus Plate Recovery Control				Human Coronavirus 229E Test Film				Human Coronavirus 229E Test Film
10−1	+	+	+	+	O	O	O	O	O	O	O	O
10−2	+	+	+	+	O	O	O	O	O	O	O	O
10−3	+	+	+	O	O	O	O	O	O	O	O	O
10−4	+	O	+	+	O	O	O	O	O	O	O	O
10−5	O	O	O	+	O	O	O	O	O	O	O	O
10−6	O	O	+	+	O	O	O	O	O	O	O	O
TCID50/1.0 mL	5.00 log10				≤0.50 log10				≤0.50 log10
TCID50/carrier	5.60 log10				≤1.10 log10				≤1.10 log10
Reduction of virus due to inactivation by test substance:					≥4.50 log10				≥4.50 log10

Recovery of 229E virus (+), or absence of both 229E virus and cytotoxicity (O) in wells containing MRC-5 host cells. 229E virus (200 µL) was previously dried on an inanimate surface, treated with a control media or test substance, neutralized and filtered, and then serially diluted in quadruplicate.