Figure 3.

Forskolin promotes VM formation of choriocarcinoma cells and decreases the recruitment of HUVECs in vitro. (A and B) Forskolin enhanced the tube formation capacity of (A) JAR and (B) JEG-3 cells, and this capacity was lower in JAR cells. (C and D) Western blotting was used to detect the expression of VE-cad at the protein level; reverse transcription-quantitative PCR was used to detected the expression of VE-cad and EphA2 at the mRNA level in (C) JAR and (D) JEG-3 cells. (E) Forskolin decreased the recruitment of HUVECs in JAR and JEG-3 cells. (F) VEGFA protein level was detected by western blot analysis. ^*P<0.05, ^**P<0.01 vs. DMSO. VM, vasculogenic mimicry; HUVECs, human umbilical vein endothelial cells; VE-cad, vascular endothelial cadherin; EphA2, ephrin-A2; VEGFA, vascular endothelial growth factor α.