Table I.
Quantitative PCR primers.
| Gene | Sequences (5'→3') |
|---|---|
| GAPDH | F: ATGGGGAAGGTGAAGGTCGG |
| R: CACGGTGCCATGGAATTTGC | |
| VE-cad | F: GCGACTACCAGGACGCTTTCA |
| R: CATGTATCGGAGGTCGATGGTG | |
| EphA2 | F: CTCTCACACCCCGTATGGCAAAG |
| R: TCCTGGTCGCCAGACATCAC | |
| MMP2 | F: TACAGGATCATTGGCTACACACC |
| R: GGTCACATCGCTCCAGACT | |
| MMP9 | F: TGTACCGCTATGGTTACACTCG |
| R: GGCAGGGACAGTTGCTTCT | |
| E-cadherin | F: GGGCTCAAGTGACTCGTAACGA |
| R: CAGCCGCTTTCAGATTTTCATC | |
| CK19 | F: AACGGCGAGCTAGAGGTGA |
| R: GGATGGTCGTGTAGTAGTGGC | |
| N-cadherin | F: ATGAAAGACCCATCCACG |
| R: CCTGCTCACCACCACTA | |
| Vimentin | F: CTTCCGCGCCTACGCCA |
| R: GCCCAGGCGAGGTACTCC | |
| ZEB1 | F: GAAAGTGATCCAGCCAAATGGA |
| R: TTTGGGCGGTGTAGAATCAGAG | |
| HESI | F: CTTCCGCGCCTACGCCA |
| R: GCCCAGGCGAGGTACTCC | |
| NICD | F: GGAGGCATCCTACCCTTTTC |
| R: TGTGTTGCTGGAGCTTCTTC |
VE-cad, vascular endothelial cadherin; EphA2, ephrin-A2; MMP, matrix metalloproteinase; CK19, cytokeratin-19; ZEB1, zinc finger E-box-binding homeobox 1; HES1, Hes family BHLH transcription factor 1; NICD, Notch 1.