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. 2020 Feb 19;56(5):1175–1185. doi: 10.3892/ijo.2020.4994

Figure 3.

Figure 3

ANKHD1 regulates the activity of the Hippo pathway. (A) Overexpression of ANKHD1 in A549 cells resulted in increased expression of total YAP and the downstream effectors CTGF and cyclin D1. Depletion of ANKHD1 in LK2 cells resulted in a decrease in total YAP, as well as CTGF and cyclin D1.(B) YAP expression was upregulated in A549 cells after ANKHD1 overexpression. Conversely, p-YAP expression was downregulated. YAP expression was downregulated after knockdown of ANKHD1 in LK2 cells but p-YAP expression was upregulated. There were no significant changes in LATS1, p-LATS1, MST, or p-MST in A549 or LK2 cells following either overexpression or knockdown of ANKHD1. ANKHD1, ankyrin repeat and KH domain-containing 1; YAP, yes-associated protein; CTGF, connective tissue growth factor; LATS, large tumor suppressor; MST, mammalian STE-20 kinase.