Figure 2.

Effects of AdoMet on cell cycle of HNSCC cells. FACS analysis of Cal-33 and JHU-SCC-011 cells treated or not (control) with 300 µM AdoMet for 24 and 48 h, respectively. FACS analysis was then performed. Representative FACS histograms of PI-stained (A) Cal-33 and (B) JHU-SCC-011 cells. On the right bar diagrams are presented showing percentage of cells in each phase of the cell cycle. Data represent the average of 3 independent experiments. The means ± SD are shown. For each sample at least 2x10⁴ events were analyzed. The protein levels of cell cycle-regulatory proteins in (C) Cal-33 and (D) JHU-SCC-011 cells treated or not (control), with 200 µM AdoMet or 300 µM AdoMet for 24 and 48 h respectively, were measured by western blot analysis and the relative densitometric analyses are reported. Data, representative of 3 experiments performed with 3 different cellular preparations, are expressed as the means + standard deviation and are reported as a percentage of protein expression of the untreated control (100%). ^*P<0.05 vs. control. The housekeeping protein, β-actin, was used as a loading control. HNSCC, head and neck squamous cell carcinoma; AdoMet, S-adenosyl-L-methionine.