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. 2020 Mar 12;56(5):1212–1224. doi: 10.3892/ijo.2020.5011

Figure 4.

Figure 4

Effects of AdoMet on the invasion of HNSCC cells on Matrigel. Invasion of (A) Cal-33 and (B) JHU-SCC-011 cells on Matrigel treated for the indicated time periods with diluents (AdoMet control) or 300 µM AdoMet, toward serum-free medium (control) or medium containing 10% FBS as a source of chemoattractant. Matrigel invasion was monitored for 24 h as changes in the Delta Cell Index (calculated as function of the Cell Index at time=0) using the xCELLigence system. On the right, slopes representing the change rate of Cell Indexes generated in the 3 to 20-h timeframe are shown. Data represent the means ± SD from 2 experiments performed in quadruplicate. **P<0.0001 vs. FBS. HNSCC, head and neck squamous cell carcinoma; AdoMet, S-adenosyl-L-methionine.