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Percentage of NS1-expressing cells also expressing SIV Gag following infection with SIV gag VLP vaccines
| SIVgag construct | % gag positive |
|---|---|
| WT | 69.3 ± 0.08 |
| DX | 22.5 ± 0.49 |
| OPT | 40.2 ± 0.43 |
| gag-pol | 87.0 ± 0.01 |
Each VLP vaccine was titred on Vero cells and cultured for 48 h. IFA was then performed using anti-NS1 and anti-SIV Gag (55-2F12) monoclonal antibodies and the IU titre calculated for each antibody. The ratio of the titres is represented as a percentage (Gag titre/NS1 titre × 100).
