. 2009 Nov 24;28(5):1253–1263. doi: 10.1016/j.vaccine.2009.11.016

Table 1.

Oligonucleotides used for cloning of the astrovirus sequences.

Name	Sequence	Location^a	Orientation	Genbank no.^b	Virus
FMDVFP1	GGGTTTTACAAACCTGTGAT G	7786-7806	Sense	DQ248888	FMDV
FMDVRP1	CCGCACACGGCGTTCACCC	7982-7920	Antisense	DQ248888	FMDV
FMDVFP2	TAAGGACTTTGTGGTCTATG
FMDVFP2Rev	CATAGACCACAAAGTCCTTA

FMDVRP2	GGCCTCGATGCTTGGGAGCC		Sense
FMDVRP2Rev	GGCTCCCA AGCATCGAGG CC		Antisense

DucPicoFP1	AGATTGATTGAAGCCTCCAGTTTG	6505-6528	Sense	AY278552	Human poliovirus 2
		7113-7136	Sense	AY563023	Duck picornavirus

DucPicoRP1	ATGSWDGTNCCHGARCABCC YGADGGCAT	6841-6869	Antisense	AY278552	Human poliovirus 2
		7458-7486	Antisense	AY563023	Duck picornavirus

DucPicoFP2	GGAAAGGAAGATGAGGGCATTG		Sense
DucPicoFP2rev	CAATGCCCTCATCTTCCTTTCC		Antisense
DucPIcoRP2	GCCAGTTTGGAGAGTATTTAC		Antisense
DucPicoRP2Rev	GTAAATACTCTCCAAACTGGC		Sense

CapFP	EcoR I		Sense
	ccGAATTCATGGCCGATAAGGCTGGGCCGC

polyT-GC	GCGCGCGCTTTTTTTTTTTTTTTTTT		Antisense

CapRP	Not I		Antisense
	ggGCGGCCGCTAGTGATGGTGATGGTGATGCTCGGCGTGGCCGCGGCTGCTAGCAGG

Location of the oligonucleotides in agreement with the sequences of the food and mouth disease virus (FMDV), duck picornavirus, and human poliovirus 2, respectively.

NCBI Genbank.