Table 1.
Reactivity of novel anti-RBD mAbs with S proteins in ELISAa
| mAb | Isotype | Antigen |
|||
|---|---|---|---|---|---|
| RBD-FC | S1-Fc | EC-S | FL-S | ||
| S6 | IgG1/k | 2.89 | 1.18 | 2.06 | 2.53 |
| S9 | IgG1/k | 2.25 | 1.33 | 2.07 | 2.28 |
| S20 | IgG1/k | 2.92 | 2.23 | 2.49 | 2.58 |
| S25 | IgG1/k | 2.82 | 1.97 | 2.27 | 2.46 |
| S29 | IgG2b/k | 2.50 | 0.87 | 0.40 | 1.62 |
| S33 | IgG2b/k | 2.55 | 0.87 | 0.58 | 1.81 |
| S37 | IgG1/k | 2.84 | 1.28 | 2.19 | 2.40 |
| S38 | IgG1/k | 2.60 | 2.12 | 2.48 | 2.44 |
| S40 | IgG1/k | 2.85 | 1.97 | 2.59 | 2.62 |
| S44 | IgG1/k | 2.85 | 2.63 | 2.42 | 2.50 |
| S50 | IgG1/k | 2.85 | 1.48 | 2.13 | 2.55 |
| S53 | IgG2b/k | 2.39 | 1.20 | 1.48 | 2.08 |
| 102D7b | IgG1/k | 0.01 | 0.01 | 2.00 | 2.75 |
Antigens were coated to ELISA plates at 0.5 μg/ml and mAbs were tested at 10 μg/ml. OD450 value >0.2 was considered positive reaction and highlighted in boldface.
102D7 is a control mAb targeting the S2 domain.