Table 2.
Mapping of anti-RBD mAbs by binding competition assaysa
| Group | Competing mAb | % Inhibition of binding by biotinylated mAb |
|||
|---|---|---|---|---|---|
| S6 | S20 | S37 | S38 | ||
| A | S6 | 92.22 | 13.41 | 94.71 | -1.52 |
| S9 | 95.78 | 19.47 | 97.55 | 8.05 | |
| S37 | 98.71 | 7.97 | 95.47 | 11.11 | |
| S40 | 99.77 | 19.83 | 99.58 | 10.13 | |
| S50 | 99.52 | 23.48 | 99.28 | 17.14 | |
| B | S20 | 27.78 | 99.77 | 24.47 | 98.96 |
| S38 | 27.45 | 98.92 | 24.85 | 99.20 | |
| S53 | 28.64 | 68.23 | 32.24 | 62.78 | |
| C | S44 | 63.31 | 52.70 | 56.94 | 16.85 |
| D | S25 | 95.06 | 83.29 | 93.56 | 85.78 |
| E | S29 | 17.88 | 11.96 | 16.15 | 6.84 |
| S33 | 13.07 | 11.64 | 24.47 | 10.19 | |
Competing mAbs were tested at 100 μg/ml for the ability to block binding of the biotinylated mAbs to the RBD-Fc in ELISA. Greater than 40% inhibition was considered positive competition (values in bold). Negative numbers indicate increased binding of the biotinylated reagent.