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. 2006 May 11;24(26):5498–5508. doi: 10.1016/j.vaccine.2006.04.054

Table 3.

Mapping of anti-RBD mAbs by binding competition assaysa

Group Competing mAb % Inhibition of binding by biotinylated mAb
10E 7 (I) 11E 12 (III) 33G4 (V) 45B5 (VI) Epitope
A S6 58.2 1.8 −2.4 0.5 Conf I-like
S9 64.2 6.1 −1.1 3.0 Conf I-like
S37 67.9 3.7 −1.8 5.2 Conf I-like
S40 92.2 7.4 2.5 19.7 Conf I-like
S50 78.8 2.3 2.3 3.6 Conf I-like



B S20 0.4 91.8 −3.6 9.0 Conf III-like
S38 −2.0 94.2 −2.1 6.8 Conf III-like
S53 20.3 90.8 1.9 −0.8 Conf III-like



C S44 6.9 −17.8 −1.2 94.7 Conf IV-like



D S25 38.9 8.5 −1.5 20.1 Conf VII



E S29 3.6 14.0 −1.3 8.0 Linear
S33 6.2 15.6 −2.9 13.7 Linear
a

Competing mAbs were tested at 100 μg/ml for the ability to block binding of the biotinylated mAbs to the RBD-Fc in ELISA. Greater than 40% inhibition was considered positive competition (values in bold). Negative numbers indicate increased binding of the biotinylated reagent.