Fig. 3.
Expression of Bovine cytokines. (A) Schematic representation of full length genomic DNA in plasmids. BAdV-3 genome (hollow box; Inverted terminal repeats (ITR). The arrows represent the direction of transcription. Early (E) regions. The name of the recombinant BAdV-3 is depicted on the right of the panel. [Construction of these viruses available on request). (B) Virus titers. Confluent monolayers of MDBK cells were infected with indicated BAdV-3. At different times post infection, the cell pellets were freeze–thawed and virus was titrated on MDBK cells. (C and D). Immunoprecipitation. Proteins from the lysates of cells (Cell) or media (Sup) of [35S] methionine labeled BAV302 (panels C and D), BAV345 (panel C) or BAV322 (panel D) infected MDBK cells were immunoprecipitated with anti-BoIFN-γ MAb 2.2.1 (panel C) or anto-BoIL-2 MAb 4F11 (panel D) and analyzed by 10% SDS-PAGE under reducing conditions.