Table 3.
Individual scores for each characteristic evaluated and overall process scores for each pathogen according to influenza virus isolation in eggs and influenza virus propagation in MDCK 33016 cells (egg > MDCK manufacturing scenario)
| Virus group | Virus family | Type/species | Individual score for each characteristic evaluated |
Process score | Rank | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| General growth properties |
Viral growth in cell substrate |
Process resistance |
|||||||||
| Growth speed and titres | Cell-free transmission | Isolation (eggs) | Propagation (MDCK 33016) | Stability | Inactivation | Splitting | |||||
| -RNA | Paramyxoviridae | Pneumovirus/hRSV | 0.5 | 0.5 | 0.1 | 0.1 | 0.5 | 0.1 | 0.5 | 0.01 | 25 |
| Metapneumovirus | 0.5 | 1 | 0.5 | 0.5 | 0.5 | 0.1 | 0.5 | 0.04 | 22 | ||
| Parainfluenzavirus | 2 | 1 | 2 | 2 | 0.5 | 0.1 | 0.5 | 0.30 | 10 | ||
| Mumps virus | 1 | 1 | 1 | 1 | 0.5 | 0.1 | 0.5 | 0.10 | 15 | ||
| Measles virus | 1 | 1 | 0.5 | 1 | 0.5 | 0.1 | 0.5 | 0.08 | 18 | ||
| +RNA | Coronaviridae | HCoV, SARS-CoV | 1 | 1 | 0.5 | 0.1 | 0.5 | 0.1 | 0.5 | 0.03 | 23 |
| Togaviridae | Rubella virus | 1 | 1 | 0.5 | 0.5 | 0.5 | 0.1 | 0.5 | 0.05 | 20 | |
| Picornaviridae | Human enterovirus | 2 | 1 | 0.5 | 0.1 | 1 | 0.3 | 1 | 0.54 | 9 | |
| Rhinovirus | 1 | 1 | 0.5 | 0.1 | 1 | 0.1 | 1 | 0.12 | 14 | ||
| ss RNA(RT) | Retroviridae | Avian retrovirus | 1 | 0.5 | 2 | 0.1 | 0.5 | 0.3 | 0.5 | 0.24 | 12 |
| Human retrovirus | 1 | 0.5 | 0.1 | 0.1 | 0.5 | 0.3 | 0.5 | 0.02 | 24 | ||
| ds RNA | Reoviridae | Mammalian reovirus | 2 | 1 | 1 | 1 | 1.5 | 0.5 | 1 | 4.50 | 4 |
| Avian reovirus | 2 | 1 | 2 | 1 | 1.5 | 0.5 | 1 | 6.75 | 1 | ||
| Birnaviridae | Avian birnavirus | 2 | 1 | 2 | 0.1 | 1.5 | 0.5 | 1 | 4.73 | 2 | |
| ss DNA | Parvoviridae | MVM | 1 | 1 | 0.5 | 0.1 | 1.5 | 0.1 | 1 | 0.18 | 13 |
| Circoviridae | Avian circovirus | 1 | 1 | 1 | 0.5 | 1.5 | 0.3 | 1 | 1.35 | 6 | |
| Porcine circovirus | 0.5 | 0.5 | 0 | 0.1 | 1.5 | 0.3 | 1 | 0.05 | 21 | ||
| ds DNA | Herpesviridae | Herpes simplex virus | 2 | 1 | 2 | 2 | 0.5 | 0.5 | 0.5 | 1.50 | 5 |
| Varicella zoster virus | 1 | 1 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.25 | 11 | ||
| EBV, CMV, HHV-6-8 | 0.5 | 0.5 | 0.5 | 0.1 | 0.5 | 0.5 | 0.5 | 0.08 | 19 | ||
| Adenoviridae | Human adenovirus | 2 | 1 | 0.5 | 0.1 | 1 | 0.5 | 1 | 0.90 | 7 | |
| Polyomaviridae | JC/BK virus, SV40 | 1 | 1 | 0.5 | 0.1 | 1.5 | 0.5 | 1 | 0.90 | 7 | |
| Avian polyomavirus | 2 | 1 | 2 | 0.1 | 1.5 | 0.5 | 1 | 4.73 | 2 | ||
| Other agents | – | Mycoplasma spp. | 1 | 0.5 | 2 | 0.1 | 0.5 | 0.1 | 0.5 | 0.08 | 16 |
| – | Chlamydia spp. | 1 | 0.5 | 2 | 0.1 | 0.5 | 0.1 | 0.5 | 0.08 | 16 | |
CMV, cytomegalovirus; ds, double-stranded; EBV, Epstein–Barr virus; HCoV, human coronavirus; HHV, human herpes virus; hRSV, human respiratory syncytial virus; MDCK, Madin–Darby canine kidney; MVM, minute virus of mice; SARS-CoV, severe acute respiratory syndrome coronavirus; ss, single-stranded; SV40, simian virus 40.