Table 2.
Anti-SARS-CoV indirect immunofluorescence assay of tracheal-lung wash fluid
| Immunized groups | The way of inoculation | Mouse number | Fluorescence intensity of IF-IgA* | ||||
|---|---|---|---|---|---|---|---|
| SARSV- alum | s.c. | 2 | − | − | |||
| SARSV | i.n. | 5 | − | − | − | − | ± |
| SARSV -CpG | i.n. | 5 | ++ | + | + | ± | ± |
| SARSV - CTB | i.n. | 5 | +++ | +++ | ++ | + | ± |
| SARSV -PEG | i.n. | 5 | ++ | ++ | ++ | ++ | ++ |
s.c.: Subcutaneous immunization, i.n.: intranasal immunization, ±: non-significant fluorescent staining, only very slight staining, +: positive fluorescent staining mainly on the membrane of infected cells, ++: positive fluorescent staining on membrane and cytoplasm, +++: straong positive fluorescent staining on membrane and cytoplasm on all cells.