Fig. 6.

Cellular immune responses were elicited by HLA-A*0201 restricted peptides 30 d after the boost vaccination. Lymphocytes were obtained from Tg mice boosted with peptides plus CpG 30 d later. (A) Expression of IFN-γ was detected by ELISA. (B) The frequency of IFN-γ-producing cells in spleens. (C) Representative results are shown from three independent experiments. Cultures were set up in triplicate. Each symbol represents one data point from one mouse. The bars represent the mean value. ^*P < 0.05; ^***P < 0.001; n.s., P > 0.05.