Table 1.
Immunization strategy.
| Groups | Immunization strategy |
|
|---|---|---|
| Prime | Boost | |
| 1 | Mock (PBS) | |
| Mock (PBS) | rTTV-E1E2 | |
| Mock (PBS) | rAAV-E1E2 | |
| 2 | pVRC-CE1E2 | |
| pVRC-CE1E2 | rTTV-E1E2 | |
| pVRC-CE1E2 | rAAV-E1E2 | |
| 3 | pAAV-CE1E2 | |
| pAAV-CE1E2 | rTTV-E1E2 | |
| pAAV-CE1E2 | rAAV-E1E2 | |
Prime–boost regimens were divided into 3 groups (3 subgroups of each, 6 mice/subgroup). The plasmid DNA prime were inoculated in 0, 2, 4 weeks with dose of 50 μg, followed by the recombinant virus boost in 20 weeks with 107 pfu of rTTV-E1E2 or 1011 vector genomes (vg) of rAAV-E1E2, respectively.