Table 2.
Comparison of neutralization titers of immune mouse sera measured by the MN and PN assays.
| MNA (CPE: mock infection: –; virus: ++++) | ||||||||
|---|---|---|---|---|---|---|---|---|
| Serum dilution | ||||||||
| Dose of virus | Immunization | 1:10 | 1:20 | 1:40 | 1:80 | 1:160 | 1:320 | 1:640 |
| 100 TCID50 | DNA/LVLPa | –b | – | – | – | ± | + | ++/+++ |
| DNA/DNAa | – | – | ± | + | ++ | +++ | ++++ | |
| PNA | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Serum dilution | ||||||||||
| Dose of pseudotypes (RLA) | Immunization | 1:10 | 1:20 | 1:40 | 1:80 | 1:160 | 1:320 | 1:640 | 1:1280 | 1:2560 |
| 1 × 106 | DNA/LVLP | 99.9c | 99.9 | 99.8 | 98.2 | 91.5 | 79.7 | 63.9 | 46.2 | 23.4 |
| DNA/DNA | 99.9 | 99.9 | 94.6 | 86.9 | 61.5 | 36.0 | 21.7 | 11.3 | 3.4 | |
| 2 × 105 | DNA/LVLP | 99.9 | 99.9 | 99.9 | 99.9 | 98.1 | 91.4 | 78.0 | 65.1 | 41.2 |
| DNA/DNA | 99.9 | 99.9 | 99.6 | 91.9 | 72.0 | 48.0 | 25.3 | 14.4 | 0.1 | |
| 4 × 104 | DNA/LVLP | 99.8 | 99.9 | 99.9 | 99.8 | 98.5 | 89.1 | 82.1 | 69.9 | 57.2 |
| DNA/DNA | 99.9 | 99.0 | 98.8 | 95.7 | 71.5 | 66.3 | 56.9 | 51.2 | 46.1 | |
a
Immunizations: DNA priming and LVLP boosting (DNA/LVLP); DNA priming and DNA boosting (DNA/DNA).
b
See Figure S3 for detailed information of scoring CPE.
c
% inhibition.