Table 3.

Neutralization titers of sera from immunized and challenged ferrets measured by the PN, MN, and HI assays.

	Immunization	Ferret no.	PN		MN titer	HI titer
			IC50	IC95
Immunized ferrets	3.75 μg HA	6283	1:80	ND	1:5	1:5
		6320	1:20	ND	1:5	1:5
		6398	1:160	ND	1:10	1:5
	30 μg HA	6243	1:320	1:40–1:80	1:40	1:40
		6278	1:160	ND	1:5	1:5
		6371	1:640–1:1280	1:80–1:160	1:10	1:20
	3.75 μg HA + AF03	6282	1:640–1:1280	1:160	1:80	1:40
		6297	1:2560–1:5120	1:320	1:80	1:80
		6304	1:640–1:1280	1:160	1:40	1:40
	30 μg HA + AlOOH	6319	1:2560–1:5120	1:320–1:640	1:640	1:80
		6397	1:2560––1:5120	1:640–1:1280	1:320	1:80
		6392	1:1280–1:2560	1:640–1:1280	1:320	1:80
	Control AF03	6329	ND	ND	1:5	1:5
		6286	ND	ND	1:5	1:5
		6303	1:40	ND	1:5	1:5
	Control AlOOH	6369	ND	ND	1:5	1:5
	Control PBS	6360	ND	ND	1:5	1:5
Challenged ferrets	3.75 μg HA	6283	1:2560–1:5120	1:2560	1:1280	1:2560
		6320	1:2560–1:5120	1:640–1:1280	1:320	1:1280
		6398	1:1280–1:2560	1:640–1:1280	1:640	1:640
	30 μg HA	6243	1:1280–1:2560	1:320–1:640	1:160	1:640
		6278	1:2560	1:640–1:1280	1:160	1:640
		6371	1:2560–1:5120	1:640	1:160	1:1280
	3.75 μg HA + AF03	6282	1:2560–1:5120	1:640–1:1280	1:1280	1:1280
		6297	1:2560	1:640–1:1280	1:320	1:640
		6304	1:5120–1:10240	1:2560–1:5120	1:1280	1:2560
	30 μg HA + AlOOH	6319	1:2560	1:320–1:640	1:320	1:640
		6397	>1:10240	1:5120–1:10240	1:2560	1:5120
		6392	1:5120–1:10240	1:1280–1:2560	1:1280	1:2560
	Control AF03	6329	1:1280	1:40–1:80	1:1280	1:2560
		6286	ND	ND	1:5	1:5
		6303	1:80	ND	1:5	1:5
	Control AlOOH	6369	1:40	ND	1:5	1:20
	Control PBS	6360	1:20	ND	1:5	1:20

ND: Not detected.