Table 4.
Neutralization titers of sera of immunized and challenged ferrets against pseudotypes expressing various H5 or H1 hemagglutinins with A/Thailand/1(KAN-1)/04/N1neuraminidase.
| HA/NA pseudotypes |
A/Thailand/1(KAN-1)/04/N1NA |
A/Cambodia/P0322095/05/N1NA |
A/Cambodia/Q0321176/06/N1NA |
H1HA A/WSN/33/N1NA |
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Clade/subclade |
1 |
1 |
1 |
– |
||||||
| Ferret no. | IC50 | IC95 | IC50 | IC95 | IC50 | IC95 | IC50 | IC95 | ||
| Immunized ferrets | 3.75 μg HA | 6283 | 1:20–1:60 | ND* | 1:60–1:180 | ND | 1:60–1:180 | ND | ND | ND |
| 6320 | ND | ND | ND | ND | ND | ND | ND | ND | ||
| 6398 | 1:60 | 1:20 | 1:180 | 1:20–1:60 | 1:180 | 1:20–1:60 | ND | ND | ||
| 30 μg HA | 6243 | 1:540 | 1:20–1:60 | 1:540–1:1620 | 1:60–1:180 | 1:1620–1:4860 | 1:180 | ND | ND | |
| 6278 | 1:180–1:540 | ND | 1:180–1:540 | 1:20–1:60 | 1:180–1:540 | 1:20–1:60 | ND | ND | ||
| 6371 | 1:540–1:1620 | 1:60 | 1:1620–1:4860 | 1:60–1:180 | 1:4860 | 1:180–1:540 | ND | ND | ||
| 3.75 μg HA + AF03 | 6282 | 1:540–1:1620 | 1:180 | 1:540–1:1620 | 1:180 | 1:1620–1:4860 | 1:540 | ND | ND | |
| 6297 | 1:1620–1:4860 | 1:180–1:540 | 1:1620 | 1:180–1:540 | 1:4860 | 1:540–1:1620 | ND | ND | ||
| 6304 | 1:540–1:1620 | 1:180 | 1:540–1:1620 | 1:180 | 1:1620–1:4860 | 1:540 | ND | ND | ||
| 30 μg HA + AlOOH | 6319 | 1:1620–1:4860 | 1:540–1:1620 | 1:4860 | 1:540 | >1:4860 | 1:1620–1:4860 | ND | ND | |
| 6397 | 1:4860 | 1:540–1:1620 | >1:4860 | 1:540–1:1620 | >1:4860 | 1:1620–1:4860 | ND | ND | ||
| 6392 | 1:1620–1:4860 | 1:540–1:1620 | 1:1620–1:4860 | 1:540 | >1:4860 | 1:1620 | ND | ND | ||
| Control AF03 | 6329 | ND | ND | ND | ND | ND | ND | ND | ND | |
| 6286 | ND | ND | ND | ND | ND | ND | ND | ND | ||
| 6303 | ND | ND | 1:20 | ND | 1:20 | ND | ND | ND | ||
| Control PBS | 6360 | ND | ND | ND | ND | ND | ND | ND | ND | |
| Control AlOOH | 6369 | ND | ND | ND | ND | ND | ND | ND | ND | |
| Challenged ferrets | 3.75 μg HA | 6283 | 1:4860 | 1:540–1:1620 | >1:4860 | 1:1620 | >1:4860 | 1:540–1:1620 | 1:540–1:1620 | 1:180–1:540 |
| 6320 | 1:1620–1:4860 | 1:180–1:540 | 1:4860 | 1:540–1:1620 | 1:1620–1:4860 | 1:540–1:1620 | 1:180–1:540 | 1:60–1:180 | ||
| 6398 | >1:4860 | 1:540–1:1620 | 1:1620 | 1:180–1:540 | 1:1620–1:4860 | 1:540–1:1620 | 1:180 | 1:20–1:60 | ||
| 30 μg HA | 6243 | 1:1620–1:4860 | 1:180–1:540 | 1:1620–1:4860 | 1:180–1:540 | >1:4860 | 1:1620–1:4860 | 1:60 | ND | |
| 6278 | 1:1620–1:4860 | 1:540–1:1620 | 1:1620–1:4860 | 1:540–1:1620 | >1:4860 | 1:1620–1:4860 | 1:540–1:1620 | 1:60 | ||
| 6371 | 1:1620–1:4860 | 1:540–1:1620 | 1:4860 | 1:540–1:1620 | >1:4860 | 1:1620–1:4860 | 1:180–1:540 | ND | ||
| 3.75 μg HA + AF03 | 6282 | 1:1620–1:4860 | 1:540 | >1:4860 | 1:1620 | 1:1620–1:4860 | 1:540–1:1620 | 1:60–1:180 | ND | |
| 6297 | 1:1620–1:4860 | 1:540–1:1620 | 1:1620–1:4860 | 1:180–1:540 | 1:1620–1:4860 | 1:540 | 1:180–1:540 | ND | ||
| 6304 | >1:4860 | 1:1620 | >1:4860 | 1:1620–1:4860 | >1:4860 | 1:1620–1:4860 | 1:540 | 1:60–1:180 | ||
| 30 μg HA + AlOOH | 6319 | 1:540–1:1620 | 1:60–1:180 | 1:1620 | 1:180–1:540 | 1:1620 | 1:180–1:540 | 1:20–1:60 | ND | |
| 6397 | >1:4860 | 1:4860 | >1:4860 | >1:4860 | >1:4860 | >1:4860 | 1:540–1:1620 | 1:60–1:180 | ||
| 6392 | >1:4860 | 1:1620–1:4860 | 1:1620–1:4860 | 1:540–1:1620 | 1:1620–1:4860 | 1:1620 | 1:180–1:540 | 1:60–1:180 | ||
| Control AF03 | 6329 | 1:180–1:540 | ND | 1:180–1:540 | 1:20–1:60 | 1:4860 | 1:60–1:180 | 1:1620 | 1:60–1:180 | |
| 6286 | ND | ND | ND | ND | ND | ND | 1:180–1:540 | 1:20–1:60 | ||
| 6303 | 1:20–1:60 | ND | 1:20–1:60 | ND | 1:60 | ND | 1:60 | ND | ||
| Control PBS | 6360 | ND | ND | ND | ND | ND | ND | ND | ND | |
| Control AlOOH | 6369 | ND | ND | 1:20 | ND | 1:20–1:60 | ND | 1:20–1:60 | ND | |
| HA/NA pseudotypes | A/Indonesia/5/05/N1NA | A/Shenzhen/406H/06/N1NA | A/Anhui/1/05/N1NA | A/Hongkong/156/97/N1NA | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Clade/subclade |
2.1 |
2.3 |
2.3 |
0 |
||||||
| Ferret no. | IC50 | IC95 | IC50 | IC95 | IC50 | IC95 | IC50 | IC95 | ||
| Immunized ferrets | 3.75 μg HA | 6283 | ND | ND | 1:20 | ND | ND | ND | 1:180 | ND |
| 6320 | ND | ND | ND | ND | ND | ND | 1:20–1:60 | ND | ||
| 6398 | ND | ND | 1:20–1:60 | ND | 1:20 | ND | 1:180 | ND | ||
| 30 μg HA | 6243 | 1:60 | ND | 1:60–1:180 | ND | 1:20–1:60 | ND | 1:60–1:180 | ND | |
| 6278 | 1:60–1:180 | ND | 1:20–1:60 | ND | 1:20–1:60 | ND | 1:60–1:180 | ND | ||
| 6371 | 1:540 | ND | 1:540 | ND | 1:60–1:180 | ND | 1:180 | 1:20–1:60 | ||
| 3.75 μg HA + AF03 | 6282 | 1:60–1:180 | 1:20 | 1:180–1:540 | 1:20–1:60 | 1:540 | 1:20–1:60 | 1:180–1:540 | ND | |
| 6297 | 1:540 | 1:60 | 1:1620 | 1:60–1:180 | 1:540–1:1620 | 1:20–1:60 | 1:180–1:540 | 1:20–1:60 | ||
| 6304 | 1:60–1:180 | 1:20 | 1:180–1:540 | 1:20–1:60 | 1:180 | 1:20 | 1:180 | ND | ||
| 30 μg HA + AlOOH | 6319 | 1:540–1:1620 | 1:60–1:180 | 1:1620–1:4860 | 1:180–1:540 | 1:1620 | 1:180 | 1:180–1:540 | 1:60–1:180 | |
| 6397 | 1:1620–1:4860 | 1:180–1:540 | 1:1620–1:4860 | 1:180–1:540 | 1:1620 | 1:180–1:540 | 1:180–1:540 | 1:180 | ||
| 6392 | 1:180–1:540 | 1:60–1:180 | 1:1620 | 1:180–1:540 | 1:540–1:1620 | 1:60–1:180 | 1:180–1:540 | 1:60–1:180 | ||
| Control AF03 | 6329 | ND | ND | ND | ND | ND | ND | ND | ND | |
| 6286 | ND | ND | ND | ND | ND | ND | ND | ND | ||
| 6303 | ND | ND | ND | ND | ND | ND | ND | ND | ||
| Control PBS | 6360 | ND | ND | ND | ND | ND | ND | ND | ND | |
| Control AlOOH | 6369 | ND | ND | ND | ND | ND | ND | ND | ND | |
| Challenged ferrets | 3.75 μg HA | 6283 | >1:4860 | 1:540–1:1620 | 1:1620–1:4860 | 1:180–1:540 | 1:540–1:1620 | 1:180–1:540 | 1:4860 | 1:180–1:540 |
| 6320 | 1:540–1:1620 | 1:60–1:180 | 1:540–1:1620 | 1:180–1:540 | 1:540–1:1620 | 1:180 | 1:540–1:1620 | 1:180–1:540 | ||
| 6398 | 1:180–1:540 | ND | 1:540 | 1:60 | 1:540–1:1620 | 1:180 | 1:540–1:1620 | 1:180–1:540 | ||
| 30 μg HA | 6243 | 1:1620–1:4860 | 1:60–1:180 | 1:540 | 1:60–1:180 | 1:180–1:540 | 1:60–1:180 | 1:540–1:1620 | 1:180–1:540 | |
| 6278 | 1:540–1:1620 | 1:180–1:540 | 1:1620 | 1:180 | 1:180–1:540 | 1:60–1:180 | 1:540–1:1620 | 1:180–1:540 | ||
| 6371 | 1:540–1:1620 | 1:180–1:540 | 1:1620–1:4860 | 1:180–1:540 | 1:1620 | 1:180–1:540 | 1:540–1:1620 | 1:180–1:540 | ||
| 3.75 μg HA + AF03 | 6282 | >1:4860 | 1:540–1:1620 | 1:4860 | 1:540–1:1620 | >1:4860 | 1:540–1:1620 | 1:1620 | 1:180–1:540 | |
| 6297 | 1:4860 | 1:180–1:540 | 1:540–1:1620 | 1:60–1:180 | 1:180–1:540 | 1:180 | 1:540 | 1:180–1:540 | ||
| 6304 | >1:4860 | 1:1620–1:4860 | >1:4860 | 1:540–1:1620 | >1:4860 | 1:540–1:1620 | >1:4860 | 1:540–1:1620 | ||
| 30 μg HA + AlOOH | 6319 | 1:540–1:1620 | 1:180–1:540 | 1:540–1:1620 | 1:60–1:180 | 1:540 | 1:60–1:180 | 1:540 | 1:60–1:180 | |
| 6397 | >1:4860 | 1:1620 | >1:4860 | 1:1620–1:4860 | >1:4860 | 1:1620–1:4860 | 1:4860 | 1:540–1:1620 | ||
| 6392 | 1:1620–1:4860 | 1:180–1:540 | 1:1620–1:4860 | 1:540–1:1620 | 1:1620–1:4860 | 1:540 | 1:1620 | 1:180–1:540 | ||
| Control AF03 | 6329 | 1:540 | ND | 1:60–1:180 | ND | 1:60 | ND | 1:540 | ND | |
| 6286 | ND | ND | ND | ND | ND | ND | ND | ND | ||
| 6303 | ND | ND | ND | ND | ND | ND | ND | ND | ||
| Control PBS | 6360 | ND | ND | ND | ND | ND | ND | ND | ND | |
| Control AlOOH | 6369 | ND | ND | ND | ND | ND | ND | 1:20 | ND | |
ND: Not detected.