Table 5.
Comparison of HI, MN, and PN assays for measuring neutralizing antibodies against HPAI H5N1 viruses.
| HI | MN | PN | |
|---|---|---|---|
| Epitope involves | Binding to RBC | Virus entry | Virus entry |
| Virus | Wild type | Wild type | Pseudotypes |
| Virus particles measured | Infectious and non-infectious | Infectious | Infectious |
| Target cells | RBC | MDCK, others | MDCK, others |
| Biocontainment requirements | BL-3 | BL-3 | BL-2 |
| Readout | HI | CPE | Luciferase activity |
| Semi-quantitative | Semi-quantitative | Quantitative | |
| Subjective | Subjective | Objective | |
| Time required for assay | Within 1 day | 5–6 days | 2–3 days |