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. 2004 Oct 30;23(11):1335–1342. doi: 10.1016/j.vaccine.2004.09.015

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Copyright © 2004 Elsevier Ltd. All rights reserved.

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Fig. 2 — Immunogenicity of induced intestinal IgA and serum IgG specific for rTGEV-S protein. Western blot was performed using intestinal lavages (lane 2) and sera (lane 4) from mice orally fed with LcS-rTGEV-S. Controls of preimmunized samples of intestinal lavage (lane 1) and serum (lane 3) were also included. Antibody binding was determined using goat anti-mouse IgA and IgG conjugated with HRP. Intestinal IgA in intestinal lavage and serum IgG bound to the 75 kDa S protein where as no band was observed on membranes probed with preimmune lavage and serum.