Table 1.
OAS1 isoforms and real-time RT-PCR conditions.
| Serial number | Isoform | GenBank accession no. | Expected protein sizes (kDa) | Real-time RT-PCR primers | Nucleotide sequences (5′ → 3′) | Annealing and extension temperature (°C) |
|---|---|---|---|---|---|---|
| 1 | p42 | NM_002534.2 | 41.7 | Forward | ACCTGAGAAGGCAGCTCACGAAAC | 60 |
| Reverse | CAGGGAGGAAGCAGGAGGTCTCAC | |||||
| 2 | p46 | NM_016816.2 | 46.0 | Forward | ACCTGAGAAGGCAGCTCACGAAAC | 68 |
| Reverse | ATCGTCTGTACTGTTGCTTTCAGCC | |||||
| 3 | p23 | AK301498.1 | 22.8 | N/T⁎ | ||
| 4 | p35 | AK300346 | 35.4 | Forward | CTCATCCGCCTAGTCAAGCAC | 60 |
| Reverse | CCAAGGCACTGTACCTGTATCC | |||||
| 5 | p44a | CF272298 | 44.1 | Forward | ACCTGAGAAGGCAGCTCACGAAAC | 66 |
| Reverse | CATTTCCACCACTTGTTAGCTGATGTC | |||||
| 6 | p52 | AY730627.1 | 52.1 | Forward | ACCTGAGAAGGCAGCTCACGAAAC | 68 |
| Reverse | ATCGTCTGTACTGTTGCTTTCAGCA | |||||
| 7 | p48 | NM_001032409.1 | 47.4 | Forward | ACCTGAGAAGGCAGCTCACGAAAC | 68 |
| Reverse | GCTGCCTGGAGTGTGCTGGGTCAG | |||||
| 8 | p44b | AJ629455.1 | 43.9 | Forward | ACCTGAGAAGGCAGCTCACGAAAC | 60 |
| Reverse | TAGTTCCTTCTGCCAACCAGTGTG |
⁎
Not tested.