Table 2.
Cytotoxicities and anrtiviral activities of curcumin conjugates 2, 3, 6, 8 and 10.
| Compound | Virus |
A |
B |
C |
D |
E |
F |
G |
H |
I |
J |
K |
L |
M |
N |
O |
P |
Q |
R |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cell | HeLa | HeLa | HeLa | HEL | HEL | HEL | HEL | HEL | Vero | Vero | Vero | Vero | Vero | CRFK | CRFK | MDCK | MDCK | MDCK | |
| 2 | EC50a | 0.011 | >0.019 | >0.019 | >0.019 | >0.038 | >0.038 | >0.038 | >0.038 | >0.038 | >0.019 | >0.019 | >0.019 | >0.019 | >0.096 | >0.096 | >0.096 | >0.096 | >0.096 |
| CC50b | 0.096 | 0.096 | 0.096 | 0.096 | 0.019 | 0.019 | 0.019 | 0.019 | 0.019 | 0.096 | 0.096 | 0.096 | 0.096 | >0.096 | >0.096 | 0.046 | 0.046 | 0.046 | |
| 3 | EC50 | 0.147 | >0.147 | >0.147 | >0.147 | >0.147 | >0.147 | >0.147 | >0.147 | >0.147 | >0.147 | >0.147 | >0.147 | >0.147 | >0.029 | >0.029 | >0.147 | >0.147 | >0.147 |
| CC50 | 0.147 | >0.147 | >0.147 | >0.147 | >0.147 | >0.147 | >0.147 | >0.147 | >0.147 | >0.147 | >0.147 | >0.147 | >0.147 | 0.146 | 0.146 | >0.147 | >0.147 | >0.147 | |
| 6 | EC50 | >0.082 | >0.082 | >0.082 | >0.082 | >0.082 | >0.082 | >0.082 | >0.082 | >0.082 | >0.082 | >0.082 | >0.082 | >0.082 | >0.03 | >0.03 | > 0.082 | >0.082 | >0.082 |
| CC50 | >0.082 | >0.082 | >0.082 | >0.082 | >0.082 | >0.082 | >0.082 | >0.082 | >0.082 | >0.082 | >0.082 | >0.082 | >0.082 | 0.011 | 0.011 | 0.082 | 0.082 | 0.082 | |
| 8 | EC50 | >0.011 | >0.011 | >0.011 | >0.011 | >0.011 | >0.011 | >0.011 | >0.011 | >0.011 | >0.011 | >0.011 | >0.011 | >0.011 | >0.011 | >0.011 | >0.011 | >0.011 | >0.011 |
| CC50 | >0.011 | >0.011 | >0.011 | >0.011 | >0.011 | >0.011 | >0.011 | >0.011 | >0.011 | >0.011 | >0.011 | >0.011 | >0.011 | >0.011 | >0.011 | >0.011 | >0.011 | >0.011 | |
| 10 | EC50 | >0.119 | >0.119 | >0.119 | >0.119 | >0.119 | >0.119 | >0.119 | >0.119 | >0.119 | >0.119 | >0.119 | >0.119 | >0.119 | 0.078 | 0.105 | >0.119 | >0.119 | >0.119 |
| CC50 | >0.119 | >0.119 | >0.119 | >0.119 | >0.119 | >0.119 | >0.119 | >0.119 | >0.119 | >0.119 | >0.119 | >0.119 | >0.119 | >0.119 | >0.119 | >0.119 | >0.119 | >0.119 | |
| Curcumin | EC50 | >0.01 | >0.01 | >0.01 | >0.01 | 0.01 | 0.01 | 0.01 | 0.01 | >0.01 | >0.01 | >0.01 | >0.01 | >0.01 | >0.271 | >0.271 | >0.271 | >0.271 | >0.271 |
| CC50 | ≥0.010 | ≥0.010 | ≥0.010 | ≥0.010 | >0.002 | >0.002 | >0.002 | >0.002 | 0.054 | 0.054 | 0.054 | 0.054 | 0.054 | >0.271 | >0.271 | >0.092 | >0.092 | >0.092 | |
| D.S-50000 | EC50 | 2 | 4 | 0.8 | – | – | – | – | – | >100 | >100 | 20 | 60 | 60 | – | – | – | – | – |
| CC50 | >100 | >100 | >100 | – | – | – | – | – | >100 | >100 | >100 | >100 | >100 | – | – | – | – | – | |
| (S)-DHPA | EC50 | 146 | >250 | 146 | – | – | – | – | – | >250 | >250 | >250 | >250 | >250 | – | – | – | – | – |
| CC50 | >250 | >250 | >250 | – | – | – | – | – | >250 | >250 | >250 | >250 | >250 | – | – | – | – | – | |
| Ribavirin | EC50 | 10 | 30 | 30 | 10 | 50 | 85 | >250 | 125 | 95 | 250 | 111 | >250 | 50 | – | – | 7 | 9 | 9 |
| CC50 | >250 | >250 | >250 | >250 | >250 | >250 | >250 | >250 | >250 | >250 | >250 | >250 | >250 | – | – | >100 | >100 | >100 | |
| Brivudine | EC50 | – | – | – | 0.08 | 50 | 2 | >250 | 50 | – | – | – | – | – | – | – | – | – | – |
| CC50 | – | – | – | >250 | >250 | >250 | >250 | >250 | – | – | – | – | – | – | – | – | – | – | |
| HHA | EC50 | – | – | – | – | – | – | – | – | – | – | – | – | – | 12.7 | 0.8 | – | – | – |
| CC50 | – | – | – | – | – | – | – | – | – | – | – | – | – | >100 | >100 | – | – | – | |
| UDA | EC50 | – | – | – | – | – | – | – | – | – | – | – | – | – | 1.3 | 2.9 | – | – | – |
| CC50 | – | – | – | – | – | – | – | – | – | – | – | – | – | >100 | >100 | – | – | – | |
| Ganciclovir | EC50 | – | – | – | 0.03 | 0.03 | >100 | >100 | 0.03 | – | – | – | – | – | >100 | 2.6 | – | – | – |
| CC50 | – | – | – | >100 | >100 | >100 | >100 | >100 | – | – | – | – | – | >100 | >100 | – | – | – | |
A, Vesicular Stomatitis virus; B, Coxsackie virus B4; C, Respiratory syncytial virus; D, Herpes simplex virus-1(KOS); E, Herpes simplex virus-2 (G); F, Vaccinia virus; G, Vesicular stomatitis virus; H, Herpes simplex virus-1TK- KOS ACV; I, Parainfluenza-3 virus; J, Reovirus-1; K, Sindbis virus; L, Coxsakie virus B4; M, Punta toro virus; N, Feline corona virus (FIPV); O, Feline herpes virus; P, Influenza A H1N1 subtype; Q, Influenza A H3N2 subtype; R, Influenza B.
50% Effective concentration (μM), or concentration producing 50% inhibition of virus-induced cytopathic effect, as determined by measuring the cell viability with the colorimetric formazan-based MTS assay.
50%Cytotoxic concentration (μM), as determined by measuring the cell viability with the colorimetric formazan-based MTS assay.