Table 2.
Oligonucleotide primers
| Primer code | Primer sequence (5′–3′)a |
|---|---|
| pQE30 forward primer | GAAGGATCCGATGCATCTGCATTCAATAAAG (BamhI) |
| pQE30 reverse primer | ACGCCTGCAGTTCGATTGGATTATCTACTTTATTA (PstI) |
| 1 | CCAAGATCTAGTCTTATAACTATACTG (BglII) |
| 2 | TCTTTTTTTTCATTTTGAGTGCCTCCTTATAATTTATTTTG |
| 3 | GGAGGCACTCAAAATGAAAAAAAAGATTATCTC |
| 4 | TGATGTGTATCAGCGTAAACACCTGACAACG |
| 5 | TGTTTACGCTGATACACATCACCATCACCATCACGGA |
| 6 | AGTCGGTACCTTATTCGATTGGATTATCTAC (KpnI) |
| 7 | GGTGATGTCCCATTTTGAGTGCCTCCTTATAATTTATTTTG |
| 8 | AGGCACTCAAAATGGGACATCACCATCACCATCACGGA |
| 9 | CCAAGATCTAACAATTGTAACCCATACAG (BglII) |
| 10 | TCTTTTTTTTCATAAAAGCGACTCCTTTCCCTCACACATCA |
| 11 | AGGAGTCGCTTTTATGAAAAAAAAGATTATCTC |
| 12 | TGATGTCCCATAAAAGCGACTCCTTTCCCTCACACAT |
| 13 | AGGAGTCGCTTTTATGGGACATCACCATCACCATCACGGA |
a
When applicable, recognition sites of restriction enzymes are underlined and enzyme name is mentioned between parentheses.