Fig. 4.

Specific cellular responses of RHDV-3A-VLP immunised pig against the vector RHDV-VLP, against the peptide 3A and against chimeric RHDV-3A-VLP at day 28. Pigs are divided in different groups, depending on the inoculation route: intranasal (IN), intramuscular + adjuvant (IM + ADJ) and intramuscular (IM). Specific RHDV-VLP and 3AT IFN-γ-producing cells are detected by ELISPOT (A). The background values (number of spots in negative control wells) were subtracted from the respective counts of the stimulated cells and the immune responses were expressed as number of spots per million of PBMCs for each animal. Shown are the results of duplicate wells of one representative experiment. Specific RHDV-VLP (B), 3A (B) and RHDV-3A-VLP (C) T-cell proliferation is detected by lymphoproliferation assay. Data are shown as SI (stimulation indexes, see Section 2) of each animal. Results of triplicate wells of one representative experiment are shown.