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Prevention of CVB3 myocarditis and protection against CVB3 challenge by intranasal immunization with VSV-based vaccine or DNA in chitosan formulations. Seven days after 3LD50 CVB3 challenge, (A) body weight loss, (B) serum CK, (C) CK-MB, (D) Viral titers in heart and (E) inflammation of HE-stained sections were measured as indicated by the arrows. The results are represented as the mean ± SD (n = 6) of three separate experiments.*P < 0.05; **P < 0.01; ***P < 0.001.
