Fig. 6.

VSV-VP1 immunization promoted MLN DCs maturation and increased proportion of polyfunctional T cells. (A) DC maturation markers CD40, CD80, CD86 and MHCII were stained and determined by flow cytometry from MLN. (B) The frequency of CD4⁺ and CD8⁺ T cells producing IFN-γ and IL-2 or TNF-α alone was analyzed after stimulation for 6 h. (C) Frequency of CD4⁺ and CD8⁺ T cells expressing two or three of IFN-γ, IL-2 and TNF-α was analyzed. (D) Proportion of total triple, double and single positive IFN-γ, IL-2 and TNF-α producing CD4⁺ and CD8⁺ T cells was shown in pie charts. The results were expressed as the mean ± SD (n = 6) from three independent experiments. ^*P < 0.05, ^**P < 0.01.