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. 2010 Oct 14;28(51):8112–8119. doi: 10.1016/j.vaccine.2010.09.106

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Copyright © 2010 Elsevier Ltd. All rights reserved.

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Fig. 2 — PCR confirmation of presence of S1 and chicken IL-18 genes in rFPVs. 1, DNA molecular Marker (DL2000); 2, S1 gene in rFPV-S1; 3, S1 gene in rFPV-S1/IL18; 4, chicken IL-18 in rFPV-S1/IL18. Also, the results were double checked by DNA sequencing done on the PCR products. DNA sequencing results showed that S1 and chicken IL-18 genes are 1 675 bp and 597 bp, respectively, and consistent with predicted results.