Table 1.

Method for establishment of hybridoma producing human monoclonal antibody against SARS CoV

Humanized monoclonal antibody against SARS-S protein
SARS TW1 strain
S protein (S431-447-KLH)
↓
Human immunoglobulin gene transchromosomic mice (KM mouse)
↓
Spleen + P3U1
↓
Hybridoma (Screening)
Humanized monoclonal antibody against SARS S (431–447) peptide: clones (21 clones)

SARS S peptide (S431-447 KLH) was immunized to KM mouse. Hybrid clones producing human monoclonal antibody against SARS S peptide were selected.