Table 1.
T cell phenotypes in Leishmania-specific peripheral blood mononuclear cells of dogs infected with L. (L.) chagasi submitted to immunotherapy with the enriched-Leishmune® vaccine
| Treatment | Dog number | Saline |
Dog number | 1 mg-Leishmune® |
||||
|---|---|---|---|---|---|---|---|---|
| CD4 (%) | CD8 (%) | CD21 (%) | CD4 (%) | CD8 (%) | CD21 (%) | |||
| ex-vivo PBMC phenotyping saline controls | 1 | 44.58 | 30.30 | 11.87 | 4 | 42.46 | 28.17 | 9.71 |
| 2 | 36.13 | 24.85 | 15.90 | 5 | 36.24 | 23.91 | 12.22 | |
| 3 | 41.57 | 32.96 | 11.66 | 6 | 37.67 | 31.45 | 9.43 | |
| 7 | 38.19 | 24.37 | 18.09 | 11 | 45.98 | 30.94 | 4.57 | |
| 8 | 38.93 | 32.69 | 16.38 | 12 | 44.19 | 27.10 | 9.09 | |
| 9 | 41.85 | 17.24 | 15.68 | 13 | 46.29 | 20.84 | 3.59 | |
| 10 | 54.35 | 23.88 | 7.96 | 14 | 42.45 | 17.38 | 15.48 | |
| 15 | 44.6 | 28.54 | 10.06 | 16 | 40.58 | 22.12 | 9.21 | |
| 17 | 37.08 | 27.97 | 11.58 | 19 | 46.91 | 31.90 | 5.89 | |
| 20 | 45.39 | 26.06 | 19.23 | 22 | 40.36 | 17.77 | 29.69 | |
| 21 | 46.31 | 23.36 | 16.23 | 23 | 40.47 | 25.50 | 24.07 | |
| 25 | 45.67 | 30.78 | 11.66 | 24 | 35.89 | 18.19 | 28.96 | |
| Mean | 42.88 | 26.97 | 13.86 | CI(95%) | 41.62 (39.38–43.86) | 24.60 (21.40–27.79) | 13.49 (8.07–18.00) | |
| in vitro Leishmania-specific lymphocytes phenotyping | 1 | 45.74 | 18.41 | 8.38 | 4 | 42.07 | 29.67 | 19.70 |
| 2 | 39.95 | 16.53 | 6.72 | 5 | 37.45 | 22.78 | 7.65 | |
| 3 | 50.42 | 34.75 | 8.49 | 6 | 43.17 | 35.62 | 14.50 | |
| 7 | 7.71 | 33.82 | 38.32 | 11 | 52.09 | 38.22 | 19.04 | |
| 8 | 16.32 | 36.09 | 15.49 | 12 | 43.87 | 33.01 | 14.32 | |
| 9 | 42.91 | 33.04 | 11.98 | 13 | 46.05 | 23.64 | 16.54 | |
| 10 | 27.65 | 41.18 | 29.55 | 14 | 46.66 | 25.41 | 26.41 | |
| 15 | 6.5 | 38.85 | 13.16 | 16 | 48.86 | 37.3 | 18.86 | |
| 17 | 50.45 | 38.35 | 23.11 | 19 | 53.61 | 37.29 | 17.76 | |
| 20 | 39.29 | 21.62 | 8.48 | 22 | 45.05 | 37.50 | 8.76 | |
| 21 | 50.61 | 23.16 | 23.58 | 23 | 49.77 | 21.59 | 10.01 | |
| 25 | 8.04 | 36.28 | 19.55 | 24 | 53.84 | 20.07 | 27.69 | |
| Mean | 32.13 | 31.01 | 17.23 | CI(95%) | 46.87 (43.93–49.80) | 30.17 (25.98–34.36) | 16.77 (13.05–20.49) | |
Data correspond to the proportions of blood peripheral mononuclear cell of saline controls or Leishmune®-treated dogs, after ex-vivo and in vitro incubation with Leishmania (L.) chagasi promastigote lysate, 12 months after infection.