Table 3.
Parasitologycal evidence of Leishmania infection in control and Leishmune® immunotherapy-treated dogs with time
| S | 6 m | 9 m | 11 m |
15 m |
L | 6 m | 9 m | 11 m |
15 m |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| BMh | BM PCR | BMh | Bh | Ln | l | S | Ln | BMh | BM PCR | BMh | Bh | Ln | L | s | Ln | ||
| 1 | − | − | − | − | − | − | − | − | 4 | − | − | − | − | − | − | − | − |
| 2 | − | − | − | − | − | − | − | − | 5 | − | − | − | − | − | − | − | − |
| 3 | − | − | − | + | − | − | − | − | 6 | D | − | − | − | − | − | − | − |
| 7 | − | + | + | D | + | Nd | Nd | Nd | 11 | − | + | + | − | + | − | + | + |
| 8 | + | + | + | − | + | − | + | + | 12 | − | − | − | − | − | − | − | − |
| 9 | + | + | − | D | − | − | − | − | 13 | + | + | + | − | − | − | − | − |
| 10 | − | − | + | − | − | − | − | − | 14 | − | − | + | + | − | − | − | − |
| 15 | − | + | + | + | + | − | + | − | 16 | + | − | D | D | − | − | − | − |
| 17 | D | − | D | − | − | − | − | − | 19 | D | − | + | − | + | − | + | + |
| 20 | − | − | D | + | + | − | + | + | 22 | − | − | − | + | − | − | − | + |
| 21 | + | − | D | D | + | − | + | + | 23 | + | + | + | − | + | + | + | + |
| 25 | − | − | − | − | − | + | − | + | 24 | − | − | − | − | − | − | − | − |
| Signs | 4 | 4 | 7 | 6 | 5 | 1 | 4 | 4 | 5 | 3 | 6 | 3 | 3 | 1 | 3 | 4 | |
| Total | 12 | 12 | 12 | 12 | 12 | 11 | 11 | 11 | 12 | 12 | 12 | 12 | 12 | 12 | 12 | 12 | |
The Leishmania infection was confirmed in saline (S) or Leishmune®-treated dogs (L) by hamster's in vivo culture of bone marrow (BMh), blood (Bh) or by Giemsa stained lymph node (Ln) samples and by PCR analysis for Leishmanial DNA in bone marrow (BM PCR). The presence of Leishmania amastigotes was confirmed after dog's autopsy in liver (l), spleen (s) and lymph nodes (Ln) by microscopy analysis of Giemsa stained smears in month 15. D = hamsters that died of clinically and parasitologycally confirmed visceral leishmaniasis. m = months after infection; Nd = not determined.