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Primers used to amplify Mafa-B, Mafa-DRB, and -DQB1 alleles
| Locus | Primer name | Primer sequence (5′ to 3′) | Temp (°C) | Product (bp) |
|---|---|---|---|---|
| Mafa-B | B-F | TGGCAGCTCTGACAGTGA | 52 | 893 |
| B-R | CTGCCTGGATAGAAACCG | |||
| Mafa -DRB | DRB1-F | TGGCAGCTCTGACAGTGA | 52 | 450 |
| DRB1-R | CTGCCTGGATAGAAACCG | |||
| Mafa -DQB1 | DQB1-F | GAAGAAGGCTTTGCGGAT | 55 | 420 |
| DQB1-R | GTCGCCGTTCCTAATAAG |
Temp, temperature.
