Fig. 4.

Antineoplastic activity. (A) Determination of inhibition ratios of L929, MCF-7, HeLa and HepG2 cells growth by MTT assay, the cells were cultured for different concentrations of CML. Values are expressed as mean ± S.D. (B) Lactate dehydrogenase (LDH) activity, L929 cells were treated with CML (1 pM) for 12, 24, 36, 48 h. The cell inhibitory ratio was measured by MTT assay, n = 5, means ± S.D. (C) Morphological observation of L929 cells. The cellular morphologic changes were observed after the cells were incubated with medium, 1 pM CML or 1 pM CML pre-incubated with Man-a(1,3:1,6)-mannotriose (2.5 mM) for 24 h under a phase contrast microscope (1, medium; 3, 1 pM CML; 5, CML pre-incubated with Man-a (1,3:1,6)-mannotriose at 37 °C for 30 min; 200 magnification) or under a fluorescence microscope by AO staining (2, medium; 4, 1 pM CML; 6, CML pre-incubated with Man-a(1,3:1,6)-mannotriose at 37 °C for 30 min; 200 magnification).