Table 1.
Specific hemagglutinating activities and yields of chromatographic fractions obtained at different steps of purification of lectin.
| Steps | Total protein (mg) | Specific hemagglutinating activity (HU/mg) | Total hemagglutination activity (HU)a | Purification foldb | Recovery of protein (%) |
|---|---|---|---|---|---|
| Crude extract | 270 | 47.4 | 12798 | 1 | 100 |
| DEAE-Sepharose fraction C | 88.5 | 128 | 11329 | 2.7 | 32.7 |
| SephacrylS-100 fraction E | 8.9 | 682 | 6069 | 14.4 | 3.2 |
The crude extract was obtained from 200 g Clematis montana Buch.-Ham.
a
Hemagglutinating unit (HU) represents the highest exhibiting complete hemagglutination. 2% rabbit erythrocytes were used for hemagglutinating activity.