Table 2.
Carbohydrate-binding specificity.
| Carbohydrate/glycoproteina | Minimum inhibitory concentration (MIC)c |
|---|---|
| D-Man | NI |
| a-Methyl-mannoside | NI |
| 2-Deoxy-d-Man | NI |
| Man-a(1,2)-Man | 40 mM |
| Man-a(1,3)-Man | 20 mM |
| Man-a(1,6)-Man | 40 mM |
| Man-a(1,3:1,6)-mannotriose | 2.5 mM |
| Yeast mannanb | 1.56 μg/ml |
Hemagglutinating inhibition test was used to study carbohydrate-binding specificity. The minimum inhibitory concentration (MIC) of sugar was exhibited in the table.
a
d-Galactose, d-glucose, maltose, d-galactosamine, d-fructose, lactose, fetuin, sucrose, N-acetyl-d-glucosamine, 2-methyl-d-glucoside, N-acetylgalactosamine and N-acetylactosamine failed to inhibit the hemagglutinating activity of CML even at 160 mM.
b
The initial concentration of yeast mannan was 4 mg/ml.
c
The minimum inhibitory concentration of sugar required for complete inhibition. NI = not inhibitory until a concentration of 160 mM.