Table 2.
Cytotoxicity and antiviral activity of compounds in HEL cell cultures.
| Compd. | Minimum cytotoxic concentrationa (μg/mL) | EC50b (μg/mL) |
||||
|---|---|---|---|---|---|---|
| Herpes simplex virus-1 (KOS) | Herpes simplex virus-2 (G) | Vaccinia virus | Vesicular stomatitis virus | Herpes simplex virus-1 TK- KOS ACVr | ||
| 2a | 100 | 1 | 1 | 0.8 | 15 | 1 |
| 2b | ≥20 | >20 | >20 | >20 | >20 | >20 |
| 2c | >100 | 9 | 8 | 9 | >100 | 9 |
| 2d | 100 | >20 | >20 | >20 | >20 | >20 |
| 2e | 100 | >20 | >20 | >20 | >20 | >20 |
| 2f | >100 | 7 | 9 | 9 | >100 | 9 |
| 2g | 100 | >20 | >20 | 12 | >20 | >20 |
| 2h | >100 | >100 | >100 | >100 | >100 | >100 |
| 2i | >100 | >100 | >100 | >100 | >100 | >100 |
| 2j | 100 | >20 | >20 | 20 | >20 | >20 |
| 2k | 100 | >20 | >20 | 20 | >20 | >20 |
| 2l | 100 | >20 | >20 | >20 | >20 | >20 |
| Brivudin (μM) | >250 | 0.04 | 50 | 4 | >250 | 250 |
| Ribavirin (μM) | >250 | >250 | >250 | >250 | 146 | >250 |
| Cidofovir (μM) | >250 | 2 | 2 | 8 | >250 | 3 |
| Ganciclovir (μM) | >100 | 0.05 | 0.07 | >100 | >100 | 2 |
Data represent mean values for three independent determinations. Variation among duplicate samples was less than 15%.
a
Required to cause a microscopically detectable alteration of normal cell morphology.
b
Required to reduce virus-induced cytopathogenicity by 50%.