Fig. 2.

Characterization of the ELVs. (A) 293T cells were transfected with either pExOVA or pSolOVA plasmids. Cells lysate (Lysate) and the pellet obtained by ultracentrifugation of the conditioned media (Pellet) were analyzed for the presence of the OVA proteins by Western blot analysis. Mock-transfected cells were used as negative controls. (B) The conditioned media from 293T cells transfected with the pExOVA plasmid was loaded onto a step gradient of sucrose with the indicated sucrose concentration and ultracentrifuged. The different sucrose fractions were collected and analyzed by Western blot for the presence of the OVA protein (upper panel) and the HSP90 protein (lower panel).