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. 2011 Jan 7;29(9):1791–1800. doi: 10.1016/j.vaccine.2010.12.106

Fig. 1.

Fig. 1

Screening of Th1 and linear immunodominant antibody-binding epitope in the S2 domain of FIPV KU-2 strain. PBMCs obtained from five FIPV KU-2-infected non-FIP cats (gray bar) and three SPF cats (open bar) were cultured with each synthesized peptide, heat-inactivated FIPV KU-2 strain as a positive control, or culture medium alone as a negative control. The concentration of fIFN-γ in the supernatants was measured using sandwich ELISA (left panel). The reactivity of plasma collected from five FIPV KU-2-infected cats (gray bar) and three SPF cats (open bar) against these peptides were examined by ELISA (right panel). The results are expressed as means ± SEM. * And **, p < 0.05 and p < 0.01, respectively, compared with the fIFN-γ levels of control culture (left panel) or with the each OD value of SPF cats (right panel).