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. 2011 Jan 7;29(9):1791–1800. doi: 10.1016/j.vaccine.2010.12.106

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Copyright © 2011 Elsevier Ltd. All rights reserved.

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Fig. 3 — Screening of Th1 and linear immunodominant antibody-binding epitope in the N protein of FIPV KU-2 strain. PBMCs obtained from five FIPV KU-2-infected non-FIP cats (gray bar), five FIPV 79-1146-infected non-FIP cats (solid bar), and three SPF cats (open bar) were cultured with individual synthesized peptide, heat-inactivated FIPV (KU-2 or 79-1146) as a positive control, or culture medium alone as a negative control. The fIFN-γ levels in the supernatants were measured (left panel). The reactivity of plasma collected from five FIPV KU-2-infected cats (gray bar), five FIPV 79-1146-infected cats (solid bar), and three SPF cats (open bar) against these peptides were examined (right panel). The results are expressed as means ± SEM. * And **, p < 0.05 and p < 0.01, respectively, compared with the fIFN-γ levels of control culture (left panel) or with the each OD value of SPF cats (right panel). N.D., not done.