Fig. 4.
Measurement of fIFN-γ specifically produced in responses to stimulation with peptides NP-7 and NP-8. PBMCs were obtained from five FIPV 79-1146-infected non-FIP cats, five FIP cats, and five SPF cats. The cells were cultured with NP-7 (dots bar), NP-8 (oblique bar), NP-13 (stripe bar) as a peptide control, heat-inactivated FIPV 79-1146 as a positive control (solid bar), or culture medium as a negative control (open bar). The concentrations of fIFN-γ were measured using sandwich ELISA. Bars represent mean ± SEM. *Indicates a significant difference compared with culture medium alone (p < 0.01).