Fig. 5.

Counting of fIFN-γ-secreting cells specifically induced by peptides NP-7 and NP-8. PBMCs were obtained from five FIPV 79-1146-infected non-FIP cats, five FIP cats, and five SPF cats. The cells were cultured with NP-7 (dots bar), NP-8 (oblique bar), NP-13 (stripe bar) as a peptide control, heat-inactivated FIPV 79-1146 as a positive control (solid bar), or culture medium as a negative control (open bar). The spot forming cells were counted using ELISpot assay. Bars represent mean ± SEM. * And **, p < 0.05 and p < 0.01, respectively, compared with culture medium alone.