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Effect of BPP9α analogues J526 and J527 on basal level of inositol phosphate production in COS-7 cells transiently transfected with BKR-B2 cDNA. Cells were prelabeled with 4 μCi/ml myo[3H]-inositol for 24 h and than stimulated with 3 × 10−8 M bradykinin for 5 min in absence or in presence of J526 (0.1 μM) or J527 (0.1 μM). Inositol phosphate formation was determined in quadruplicates. Shown are the mean ± S.E.M. of four independent comparative experiments.
