Table 3.

Antiviral evaluation of compounds 1–33 in Vero cell cultures.

Compound	Antiviral activity: EC50a (μM)						Cytotoxicity: MCCb (μM)
	Para-influenza-3 virus	Reovirus-1	Sindbis virus	Coxsackie B4 virus	Punta Toro virus	Yellow fever virus
1	>100	>100	>100	>100	>100	>100	>100
2	>100	>100	>100	>100	>100	>100	>100
3	>100	>100	>100	>100	>100	>100	>100
4	>100	>100	>100	>100	>100	>100	>100
5	>100	>100	>100	>100	>100	>100	>100
6	>100	>100	>100	>100	>100	>100	>100
7	>100	>100	>100	>100	>100	>100	>100
8	>100	>100	>100	>100	>100	>100	>100
9	>100	>100	>100	>100	>100	>100	>100
10	>100	>100	>100	>100	>100	>100	>100
11	>100	6.8	>100	>100	>100	>100	≥100
12	>100	9.5	>100	>100	>100	>100	>100
13	>100	2.1	>100	>100	>100	>100	>100
14	>100	6.8	>100	>100	>100	>100	≥100
15	>100	>100	>100	>100	>100	>100	>100
16	>100	3.8	>100	>100	>100	>100	≥100
17	>100	>100	>100	>100	>100	>100	>100
18	>100	>100	>100	>100	>100	>100	>100
19	>100	>100	>100	>100	>100	>100	>100
20	>100	>100	>100	>100	>100	>100	>100
21	>100	>100	>100	>100	>100	>100	>100
22	>100	>100	>100	>100	>100	>100	>100
23	>100	>100	>100	>100	>100	>100	>100
24	>100	>100	>100	>100	>100	>100	>100
25	>100	>100	>100	>100	>100	>100	>100
26	>100	>100	>100	>100	>100	>100	>100
27	>100	>100	>100	>100	>100	>100	>100
28	>100	>100	>100	>100	>100	>100	>100
29	>100	>100	>100	>100	>100	>100	>100
30	>100	>100	>100	>100	>100	>100	20
31	>100	>100	>100	>100	>100	>100	>100
32	20	5.4	8.9	46	>100	>100	≥20
33	>100	>100	>100	>100	>100	>100	>100
DS-10,000c	>100	>100	2.3	2.3	45	0.40	≥100
Ribavirin	112	>250	>250	>250	29	>250	>250
Mycophenolic acid	0.40	1.8	1.4	>100	20	2.3	>100

^aEC₅₀: 50% effective concentration producing 50% inhibition of virus-induced cytopathic effect (CPE), as determined by microscopy.

^bMCC: minimum compound concentration producing a microscopically detectable alteration in normal cell morphology.

^cFor DS-10,000 (dextran sulphate of MW 10,000) concentrations are in μg/ml. Values shown are the mean of two determinations.