Table 4.
Drop in anti-RSV activity of compound 14 when combined with dihydrofolic acid.
| Concentration of dihydrofolic acid (μM) | Antiviral activity of compound 14 |
Cytotoxicity of compound 14 |
|
|---|---|---|---|
| EC50a (μM) | Fold-increaseb | MCCc (μM) | |
| 400 | 0.80 | 267 | 20 |
| 40 | 0.40 | 133 | 20 |
| 16 | 0.090 | 30 | 20 |
| 6.4 | 0.030 | 10 | 20 |
| 2.6 | 0.006 | 2 | 20 |
| 1.0 | 0.004 | 1 | 20 |
| 0.4 | 0.003 | 1 | 20 |
| 0 | 0.003 | 1 | 20 |
a
EC50: 50% effective concentration producing 50% inhibition of virus-induced cytopathic effect (CPE), as determined by microscopy.
b
Ratio of EC50(i) to EC50(0).
c
MCC: minimum compound concentration producing a microscopically detectable alteration in normal cell morphology. Values shown are the mean of three determinations.