Table 3.
MVA-CR19 production in CR.pIX suspension cells in shake flasks for different feeding strategies after seed virus addition.*
| Mode | Harvest vol.a [mL] | Harvest titer [virions/mL] | Max. cells [×109] | Pooled titerb [virions/mL] | Yv/cell [virions/cell] | PV [virions/(L × d)] |
|---|---|---|---|---|---|---|
| FB | 100 | 3.7 × 109 | 1.9 | 3.7 × 109 | 2.0 × 102 | 1.6 × 1011 |
| DME | H1: 45 | 1.6 × 108 | 4.0 | 3.8 × 109 | 1.3 × 102 | 1.7 × 1011 |
| H2: 45 | 5.6 × 108 | |||||
| H3: 50 | 1.0 × 1010 | |||||
| F+D | H1: 45 | 1.0 × 108 | 1.9 | 5.3 × 109 | 2.7 × 102 | 2.6 × 1011 |
| H2: 50 | 1.0 × 1010 | |||||
*
All data correspond to values obtained at/up to 72 hpi (time of highest virus titer and productivity yields).
a
H: harvest.
b
Titer of accumulated harvests.