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. 2007 Jan 22;25(18):3599–3605. doi: 10.1016/j.vaccine.2007.01.055

Fig. 1.

Fig. 1

Detection of FliC production and surface-display by L. casei transformants. (a) Immunoblotting of total cell extract (CE) and culture supernatant (Sup) of L. casei carrying pLP401∷FliC (LCF) and pLPEmpty (LCN). An amount equivalent to 5 × 107 cfu of bacteria was loaded into each lane, and 50, 25, 12.5, and 6.25 ng of purified FliC were included as a standard. The sizes of the molecular mass markers are shown in the left margin. (b) Flow cytometric analysis of the L. casei transformants. Ten thousand particles were analyzed and fluorescence levels (FL1-H) from bacterial cells are shown in histogram form.